Forkhead Box Protein O5 (FOXO5)

zfoxo5 RNA transcriptswere maternal-inherited and persistently expressed thereafteruntil 48 hpf. Using whole-mount insitu hybridization, zfoxo5 expression was observed ubiquitouslyexpressed in early stage of zebrafish embryogenesis whereaszfoxo5 transcripts were found in hindbrain and heart at 48 HPF.Defected developmental morphology such as reduced body length,abnormality of brain and eye development, and curved of bodywas observed in embryos injected with morpholino antisense oligonucleotides(MO) against zfoxo5 but not control or 5-base mismatch morpholino.The characterized phenotype of zfoxo5 morphants can be rescuedby injected with zfoxo5 mRNA. Downregulation of zfoxo5 triggeredapoptosis in brain and trunk region. Through phosphorylationby AKT leaded to cytoplasma retention of zfoxo5. zFOXO5 may play an important rolein zebrafish development and might be regulated by AKT pathway.