Cleavage Stimulation Factor 64kDa Subunit Tau Variant (CSTF2t)

By sequencing clones obtained from a size-fractionated brain cDNA library, Ishikawa et al. (1998) cloned CSTF2T, which they designated KIAA0689. RT-PCR analysis detected moderate expression in all tissues examined.Using the RNA-binding domain of mouse Cstr2t as bait, Dass et al. (2002) cloned human CSTF2T from a testis cDNA library. The deduced 616-amino acid protein has a calculated molecular mass of 64.4 kD. It has an N-terminal RNA-binding domain, 9 MEARA/G repeats, and a highly conserved C-terminal domain. Human CSTF2T shares 89.8% amino acid identity with mouse Cstf2t, which contains 630 amino acids and has 8 MEARA/G repeats. Human CSTF2T and CSTF2 share 74.9% amino acid identity.