Immune Receptor Expressed On Myeloid Cells 2 (IREM2)

CD300LE is an activating receptor of the immunoglobulin (Ig) superfamily expressed on myeloid cells. It mediates activating signals by interacting with DAP12. The predicted 205-amino acid type I transmembrane protein has a 12-residue signal peptide, followed by a 44-amino acid extracellular region, a transmembrane domain, and a 10-amino acid cytoplasmic tail. The extracellular region contains an Ig variable domain and a membrane proximal region with a potential N-glycosylation site, and the transmembrane domain contains a charged residue (lys). RT-PCR and FACS analysis showed that IREM2 expression was restricted to dendritic cells and monocytes and was downregulated upon differentiation. Immunoprecipitation analysis revealed expression of a 34-kD IREM2 protein that was reduced to 31 kD by N-deglycosylation.