Lck Interacting Transmembrane Adaptor 1 (LIME1)

LIME1 is a raft-associated transmembrane adaptor phosphoprotein that is preferentially expressed in hemopoietic cells, particularly T cells.The predicted 295-amino acid type III protein has a 4-amino acid extracellular peptide, a 23-amino acid transmembrane domain that is followed immediately by a potential palmitoylation motif (CxxC) and 2 arg residues, and a cytoplasmic domain with 5 potential tyrosine phosphorylation sites. RT-PCR analysis detected expression of LIME in liver, thymus, peripheral blood lymphocytes (PBLs), and other lymphoid tissues. within PBLs, LIME was predominantly expressed in CD4 and CD8 T cells, and it was downmodulated after T-cell activation. Confocal microscopy, biosynthetic labeling, and Western blot analysis demonstrated membrane expression and association with lipid rafts.