Nedd4 Family Interacting Protein 1 (NDFIP1)

NDFIP1 belongs to a small group of evolutionarily conserved proteins with three transmembrane domains. It is a potential target for ubiquitination by the Nedd4 family of proteins. This protein is thought to be part of a family of integral Golgi membrane proteins.The deduced 221-amino acid NDFIP1 protein has a calculated molecular mass of 26 kD and contains 2 N-terminal PY motifs and 3 predicted transmembrane regions. Northern blot analysis of human tissues detected expression in most adult tissues with high expression in adult brain tissues, such as cerebellum, pituitary, and thalamus, and kidney, liver, testis, salivary gland, and adult placenta. High expression was also detected in fetal brain, kidney, and lung. Immunofluorescence studies and subcellular fractionation localized NDFIP1 to the Golgi complex.