Phytanoyl Coenzyme A-2-Hydroxylase Interacting Protein (PHYHIP)

The deduced 330-amino acid protein has a calculated molecular mass of 38 kD. Mouse and human PHYHIP share 99% amino acid identity, and both contain 1 putative tyrosine kinase site and 3 N-glycosylation sites. Northern blot analysis detected a 3.4-kb transcript expressed only in human brain. Northern blot analysis of mouse tissues showed variable expression of Phyhip during development. At embryonic day 18, Phyhip was expressed at a high level in skin and at low levels in all other tissues examined. At neonatal day 1, higher levels were detected in both brain and skin, and adult animals expressed Phyhip only in brain. In situ hybridization of rat brain showed expression localized to the supragranular layer in the cerebral cortex, dentate gyrus, hippocampus, Purkinje cell layer, and several central nuclei.