Protein Regulator Of Cytokinesis 1 (PRC1)

PRC1 encodes a protein that is involved in cytokinesis. The encoded protein is at high level during S and G2/M and drop dramatically after cell exit mitosis and enter G1. It is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). PRC1 was a good substrate for several cyclin-dependent kinases (CDK) in vitro and was phosphorylated in vivo at sites that were phosphorylated by CDK in vitro, suggesting that PRC1 is an in vivo CDK substrate. Microinjection of anti-PRC1 antibodies into HeLa cells blocked cellular cleavage, but not nuclear division, indicating a functional role for PRC1 in cytokinesis.