Rho GTPase Activating Protein 24 (ARHGAP24)

The predicted p73 protein contains 655 amino acids and has a calculated molecular mass of 73 kD. The deduced 655-amino acid protein has a calculated molecular mass of 72 kD and shares 87% amino acid identity its mouse homolog. Northern blot analysis detected variable expression of Rcgap72 in all mouse tissues examined, with highest expression in kidney. Northern and Western blot analyses of several human cell lines detected RCGAP72 expression in HeLa cells and a nonsmall cell lung carcinoma cell line only. Western blot analysis of HeLa cells revealed an 80-kD RCGAP72 protein. Immunofluorescence microscopy of transfected mouse fibroblasts showed fluorescence-tagged RCGAP72 associated with actin stress fibers. In