Src Like Adaptor (SLA)

The SLA was identified by exon trapping on overlapping cosmids encompassing the largest TG intron. A 2.6-kb transcript, with the highest levels of expression in fetal brain and lung, was detected on Northern blots. Two full-length cDNAs (1 alternatively spliced) were isolated from a fetal brain library, both containing an open reading frame of 276 amino acids but lacking a catalytic tyrosine kinase domain. The gene showed a high degree of cross-species similarity and appeared to be transcribed in the direction opposite to TG. They symbolized the gene SLAP. The predicted 281-amino acid protein has both SH3 and SH2 adaptor motifs similar to those in the Src family of nonreceptor tyrosine kinases but had no catalytic domain. The protein was named Slap by the authors. Recombinant Slap was shown to bind to activated Eck receptor tyrosine kinase.