Telomeric Repeat Binding Factor 2 Interacting Protein (TERF2IP)

The RAP1 cDNA is identical to the KAIA804 cDNA reported by the NEDO Japanese sequencing project. The predicted 47-kD RAP1 protein contains 399 amino acids. A motif search revealed that RAP1 has an N-terminal BRCT domain and a central Myb-type helix-turn-helix motif. RAP1 also has an acidic C terminus (amino acids 214 to 382; pI around 3.8) featuring a predicted 33-amino acid coiled-coil region and a bipartite nuclear localization signal. Sequence alignments showed an additional region of sequence similarity in the C termini of yeast and human RAP1 that coincides with the main protein-protein interaction domain of S. cerevisiae Rap1. Thus, human RAP1 has 3 conserved sequence motifs in common with yeast Rap1.Northern blot analysis detected ubiquitous expression of a 2.5-kb RAP1 transcript.