Calcium Regulated Heat Stable Protein 1 (CARHSP1)

CARHSP1 protein contains 147 amino acids and is composed of nearly 14% proline. It is ubiquitously expressed and may play a common role in calcium mediated signal transduction.This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence.