Cell Cycle Exit And Neuronal Differentiation 1 (CEND1)

The deduced 149-amino acid BM88 protein has a calculated molecular mass of about 15 kD. It has a central proline-rich region containing 4 PxxP motifs, which typically bind SRC homology-3 (SH3) domains, a putative C-terminal transmembrane region, and several potential sites for N-glycosylation, myristoylation, and phosphorylation. BM88 shares 79.9% and 76.4% amino acid identity with mouse and porcine Bm88, respectively. Northern blot analysis detected a 1.8-kb transcript expressed in mouse and human whole brain and in all specific human brain regions examined. Much less BM88 was expressed in spinal cord, and no expression was detected in HeLa cells. Western blot analysis determined that the BM88 protein has an apparent molecular mass of about 23 kD.