Cold Shock Domain Protein A (CSDA)

Cold-shock domain (CSD) proteins are characterized by a highly conserved central domain of approximately 100 amino acids that is homologous to bacterial cold-shock proteins.The deduced 342-amino acid DBPA protein has a cold-shock domain and a DNA-binding domain. The authors isolated the DBPA genomic sequence and found that it contains 10 exons spanning 24 kb; exon 6, which encodes 69 amino acids, is alternatively spliced. Northern blot analysis of human tissues demonstrated highest levels of DBPA transcription in skeletal muscle and heart. Immunofluorescence detected DBPA protein expression in both the cytoplasm and nucleus of HeLa cells.