Ecotropic Viral Integration Site 2B (EVI2B)

EVI2B locus lies within the same intron of the NF1 gene as EVI2A and contains a 57-bp 5-prime exon that is noncoding, an 8-kb intron, and a 2,078-bp 3-prime exon that includes the entire open reading frame. EVI2B is transcribed in the same direction as EVI2A; its 5-prime exon lies only 4 kb downstream from the 3-prime exon of the EVI2A locus.Evi2b, lies approximately 2.8 kb from the 3-prime end of Evi2a, in the midst of a cluster of viral integration sites identified in retrovirus-induced myeloid tumors; thus, EVI2B may function as an oncogene in these tumors.Sequencing studies predicted an EVI2B protein of 448 amino acids that is proline-rich and contains an N-terminal signal peptide, an extracellular domain with 4 potential glycosylation sites, a single hydrophobic transmembrane domain, and a cytoplasmic hydrophilic domain.