Ecotropic Viral Integration Site 5 (EVI5)

The deduced 810-amino acid protein shares 88% identity with mouse Evi5. A 200-amino acid region of NB4S shares significant homology with TBC1 box motif proteins involved in cell growth and differentiation. The C-terminal end of the protein contains a number of coiled-coil domains, indicating a possible protein-protein binding function. Northern blot analysis detected a 7.5-kb transcript in a wide variety of tissues.Immunofluorescence microscopy localized EVI5 predominantly to centrosomes in interphase human osteosarcoma cells, and centrosomal localization required the C-terminal half of EVI5. In synchronized HeLa cells, EVI5 accumulated in early G1 phase, reached maximal level by late G1, and decreased in early mitosis concomitantly with EMI1 in a ubiquitin- and proteasome-dependent manner.