High Mobility Group Protein 20B (HMG20B)

High mobility group (HMG) proteins are nonhistone chromosomal proteins that contain a conserved DNA-binding domain called the HMG box.BRAF35 protein contains a nonspecific DNA-binding HMG domain and a kinesin-like coiled-coil domain. The only protein with structural domains similar to those of BRAF35 was BAF57, a subunit of the SWI/SNF complex. In situ hybridization showed that similar to BRCA2, BRAF35 mRNA expression levels in mouse embryos were highest in proliferating tissues with high mitotic index. Strikingly, nuclear staining revealed a close association of the BRAF35-BRCA2 complex with condensed chromatin coincident with histone H3 phosphorylation. Antibody microinjection experiments suggested a role for the BRCA2-BRAF35 complex in modulation of cell cycle progression.