Neutrophil Cytosolic Factor 1 (NCF1)

Neutrophil cytosol factor 1 is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.A predominant transcriptional start site was identified 21 nucleotides upstream of the translation initiation codon. Transient transfections of HL60 human myeloid cells were performed with a series of 5-prime-deletion p47-phox-luciferase reporter constructs to identify the gene promoter sequences. The -224 and -86 constructs had the strongest p47-phox promoter activity, whereas the -46 construct showed a major reduction in activity and the -36 construct a complete loss of activity.