Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor Like Protein 2 (NFkBIL2)

IKBR cDNA encodes a deduced 481-amino acid protein containing 3 ankyrin-repeat motifs. Northern blot analysis revealed an approximately 5.5-kb transcript in lung and trachea epithelial but not fibroblast cells. In whole tissues, the transcript was detected in skeletal muscle and heart but not in lung (where epithelial cells constitute only 10% of adult alveolar epithelium), liver, kidney, pancreas, brain, or placenta. Western blot analysis demonstrated a 52-kD protein in HeLa cell lysates. In electrophoretic mobility shift assays, DNA binding by NFKB/RELA was inhibited in the presence of NFKBIL2.NFKBIL2 gene contains 13 exons, spanning 6,550 bp of genomic sequence. They reported a revised mRNA and protein sequence for IKBR, which predicted that the protein is larger than originally described.