Sialic Acid Binding Ig Like Lectin 11 (SIGLEC11)

SIGLEC11 has conserved arginine and aromatic amino acids at its N terminus and 3 conserved cysteines in the first and second Ig-like domains. An aromatic amino acid conserved among all other SIGLECs within beta strand G is replaced by a histidine in SIGLEC11. No SIGLEC11-positive cells were found by flow cytometry of peripheral blood leukocytes. Immunohistochemical analysis of human tissue sections revealed expression in Kupffer cells in liver, intestinal lamina propria macrophages, brain microglia, perifollicular cells of the spleen, and intense staining of chronically inflamed tissues. SIGLEC11 stably expressed in a mouse macrophage cell line could be immunoprecipitated as a 100-kD band under reducing conditions and as a 180-kD band under nonreducing conditions, suggesting that it exists in the membrane as a disulfide-linked dimer.