TGF Beta Inducible Early Response Gene 2 (TIEG2)

TIEG2, a novel, ubiquitously expressed SP1-like zinc finger protein related to TIEG1. The authors isolated TIEG2 cDNAs from a human epithelial CF Pac-1 cDNA library. The predicted 512-amino acid, 55.24-kD protein has 3 SP1-like zinc finger motifs at the C terminus and a proline-rich N terminus. TIEG2 and TIEG1 are 91% homologous within the zinc finger motifs and 44% homologous outside these domains.mouse and human TIEG2 share 98% amino acid identity. Residues within each of the 3 zinc fingers that are critical for DNA binding are completely conserved in mouse and human TIEG2.TIEG2 is a nuclear protein that can specifically bind to a consensus SP1-like binding site in vitro and can repress a promoter containing Sp1-like binding sites in transfected cells. TIEG2 overexpression in cultured epithelial cells inhibited cell proliferation.