Tax1 Binding Protein 1 (TAX1BP1)

TAX1BP1 binds TRAF6 but not TRAFs 1-5; the interaction requires the N-terminal zinc and ring finger domains of TRAF6 and either the N-terminal first coiled-coil region or the 2 coiled-coil regions of the C-terminus of TAX1BP1. Immunoprecipitation and immunoblot analysis determined that stimulation of cells with IL1B, but not with TNF or CD40LG, induces independent, mutually exclusive TRAF6/TAX1BP1 complexes as well as TRAF6/IRAK complexes. Transient expression of TRAF6, but not TAX1BP1, induces NFKB and JNK (MAPK8) activation.TXBP151, encoding a 747-amino acid protein. Northern blot analysis revealed expression of 1.9- and 2.4-kb transcripts in various cell lines. Western blot analysis showed that TAX1BP1 is expressed as an 86-kD or a 68-kD protein.