Afadin And Alpha Actinin Binding Protein (ADIP)

SSX2IP gene contains 14 exons and spans more than 46 kb. The first exon is embedded in a predicted CpG island, and there is no consensus TATA box, characteristics of a housekeeping gene. The deduced 614-amino acid protein has a calculated molecular mass of 71 kD. SSX2IP contains 4 coiled-coil domains, a putative phosphate-binding P loop, and a bipartite nuclear localization signal. It also has potential sites for N-glycosylation, phosphorylation, N-myristoylation, and amidation. Northern blot analysis of adult and fetal tissues detected ubiquitous expression of 4.0-, 6.0-, and 6.7-kb transcripts, with highest expression in adult brain. SDS-PAGE of in vitro transcribed and translated SSX2IP cDNA revealed doublet protein bands with apparent molecular masses of 64 and 68 kD.