Calmodulin Like Protein 6 (CALML6)

By EST database searching with the sequence of the 4-EF hand-containing calglandulin protein in Bothrops insularis snake as query, followed by PCR analysis, Chen et al. (2004) cloned human CALML6, which they called CAGLP, from skeletal muscle.The deduced 181-amino acid protein contains 4 EF-hand motifs and shares 80% sequence identity with the snake protein. RT-PCR analysis detected a single transcript at highest levels in skeletal muscle but also in prostate, thymus, heart, bone marrow, and ovary. Western blot analysis of a CAGLP-EGFP fusion product in HeLa cells showed that CAGLP has a molecular mass of 19 kD. Fluorescence microscopy localized the CAGLP-EGFP fusion protein throughout the cell, with a higher accumulation in cytoplasm than in the nucleus.