Myopalladin (MYPN)

MYPN is a component of the sarcomere that tethers nebulin in skeletal muscle and nebulette in cardiac muscle to alpha-actinin at the Z lines.The deduced 1,320-amino acid protein has a calculated molecular mass of 145 kD. It contains 5 immunoglobulin (Ig) repeats and 6 interdomain insertions. RNA dot blot analysis of multiple human tissues detected expression restricted to adult skeletal muscle and adult and fetal heart; fetal skeletal muscle was not examined. Immunofluorescent staining revealed that rat Mypn localized at the Z line of both heart and skeletal myofibrils, and it colocalized with cardiac ankyrin repeat protein (Carp) within the I band. Mypn localized to the nucleus in about 75% of isolated rat cardiac myocytes.