G Protein Coupled Receptor 25 (GPR25)

Using degenerate PCR of genomic DNA with primers based on the sequence of SLC1, Jung et al. (1997) identified a novel G protein-coupled receptor, GPR25. The GPR25 gene has no introns and encodes a polypeptide of 360 amino acids. The predicted protein contains 7 putative transmembrane domains. Its closest homolog is GPR15. Jung et al. (1997) used fluorescence in situ hybridization to map the GPR25 gene to human chromosome 1q32.1, in close proximity to several other G protein-coupled receptors. G protein-coupled receptors are found only in eukaryotes, including yeast, choanoflagellates, and animals. The ligands that bind and activate these receptors include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters, and vary in size from small molecules to peptides to large proteins.