Benzisothiazolinone (BIT)

Benzisothiazolinone (BIT) is a widely used biocide and belongs to the group of isothiazolinones. Benzisothiazolinone has a microbicide and a fungicide mode of action. It is used as a preservative in emulsion paints, varnishes, adhesives, washing agents, fuels and in the papermaking process. In paints, it is commonly used as a mixture with methylisothiazolinone. Benzisothiazolinone as a useful template for the design of new monoacylglycerol lipase inhibitors: investigation of the target residues and comparison with octhilinone. The regulation of 2-arachidonoylglycerol (2-AG) levels is a major issue as 2-AG has been proven to participate in numerous physiopathological phenomena such as neuroprotection or analgesia. Octhilinone, a cysteine-reagent compound, has recently been shown to inhibit in the nanomolar range monoacylglycerol lipase (MAGL), the major enzyme responsible for the degradation of 2-AG.