Dolichyl Beta-D-Glucosyl Phosphate (Dol-P-Glc)

Dolichyl β-D-glucosyl phosphate is a molecule involved in glycosylation. It is a polyprenyl glycosyl phosphate having dolichol as the polyprenyl component and β-D-glucose as the glycosyl component. Enzymatic glucosylation of dolichol monophosphate (dolichol-P) from UDP-D-[3H]glucose was studied using the microsomal fraction of BHK-21 cells. The reaction product was separated by preparative thin-layer chromatography, further purified by DEAE-cellulose acetate column chromatography, and characterized as dolichyl-beta-D-glucosyl phosphate (Dol-P-Glc). The microsomal fraction of BHK cells catalyzed the incorporation of glucose from UDP-[3H]glucose into ceramides (endogenous and exogenous) and Dol-P; both reactions required Mn2+. Maximal glucosylation of Dol-P was achieved at pH 5.6-5.8 in the presence of a non-ionic detergent, Zonyl A.