Centrobin (CNTROB)

CNTROB is a centrosomal protein that interacts with BRCA2 and is required for centriole duplication and cytokinesis.The authors identified transcripts encoding a deduced 903-amino acid predominant isoform called centrobin-alpha and a deduced 925-amino acid minor isoform, centrobin-beta. CNTROB contains a predicted central coiled-coil region with N- and C-terminal noncoiled regions, a structure common to centrosomal proteins pericentrin (PCNT1) and ninein (NIN). Immunofluorescence studies using the normal mammary epithelial cell line 76N and several cancer cell lines localized CNTROB to 1 or 2 perinuclear dots in interphase cells and to a single focus at the end of each mitotic spindle in mitotic cells, in a pattern colocalizing with that of centrosomal protein gamma-tubulin (TUBG1).