Dynein, Light Chain, LC8-Type 2 (DYNLL2)

Dlc2 encodes an 89-amino acid protein that shows 93% and 100% identity to rat Dlc1 (DNCL1) and human DLC2, respectively. Coimmunoprecipitation and GST pull-down assays confirmed that Dlc2 binds with Gph, and yeast 2-hybrid analysis showed that Dlc2 binds to residues 181-243 of Gph, in the central linker domain.In primary hippocampal neurons, Dlc2 localized to the cytoplasm in a granular staining pattern and to the plasma membrane of dendrites and cell bodies in a punctate staining pattern. The pattern of Dlc2 membrane staining overlapped that of synaptophysin and partially overlapped that of Gph. Electron microscopy of spinal cord neurons showed that Dlc2 localizes to the edges of postsynaptic differentiations, along cytoskeletal structures, and at the edge of the Golgi apparatus.