Fatty Acid Binding Protein 5, Epidermal (FABP5)

Fatty acid binding protein 5 was microsequenced, and degenerate oligonucleotides based on the amino acid sequence were used to screen a lambda-gt11 cDNA library prepared from noncultured psoriatic keratinocytes. The PAFABP cDNA encodes a 135-amino acid protein with molecular weight 15,164. By Northern blot analysis, the authors detected elevated levels of PAFABP message in noncultured psoriatic keratinocytes as compared to normal keratinocytes. PAFABP mRNA was also observed in transformed human epithelial cell lines, A431 epidermoid carcinoma cells, transformed human amnion (AMA) cells, and lymphoid Molt 4 cells. PAFABP protein was increased in primary normal keratinocytes induced to differentiate, as determined by 2D gel electrophoresis.