Low Density Lipoprotein Receptor Related Protein 1B (LRP1B)

LRP1B belongs to the low density lipoprotein (LDL) receptor gene family. full-length LRP1B was unstable following transfection in Lrp1 null Chinese hamster ovary (CHO) cells. In order to characterize the ligands for LRP1B, generated a minireceptor of LRP1B that could be stably expressed in CHO cells. The minireceptor began with the EGF precursor-like repeat before the fourth cluster of ligand-binding repeats (domain IV) and contained ligand-binding domain IV, the transmembrane domain, and the cytoplasmic tail. Ligands for the minireceptor, including receptor-associated protein , urokinase plasminogen activator, tissue-type plasminogen activator , and plasminogen activator inhibitor-1, were bound, internalized, and degraded via their association with the minireceptor.