Melanoma Antigen Family F1 (MAGEF1)

Stone et al. (2001) cloned MAGEF1 from an ovarian tumor cDNA library. The deduced 308-amino acid protein contains a C-terminal MAGE motif and shares extensive homology with the MAGE superfamily of tumor antigens. By EST database analysis, Stone et al. (2001) identified MAGEF1 clones containing either 6 or 7 GGA trinucleotide microsatellite repeats encoding glutamic acids. They also identified a separate set of 2 GGAs encoding 2 glycine resides. The trinucleotide repeats appeared to be unique to MAGEF1. Northern blot analysis detected a 1.7-kb transcript in normal ovary and peripheral blood leukocytes and in 6 of 7 ovarian tumors examined. RNA dot blot analysis detected MAGEF1 expression in all tissues examined.Stone et al. (2001) determined that a single exon contains the coding region of the MAGEF1 gene.