Myozenin 3 (MYOZ3)

The predicted 251-amino acid protein exhibits highest homology to MYOZ1 and MYOZ2 at its N and C termini and shares 75% identity with mouse Myoz3. Northern blot analysis of adult human tissues revealed expression of a major 3.4-kb transcript, as well as 2 other transcripts, in skeletal muscle only,Analysis of deletion mutants revealed 2 independent domains that are sufficient to bind ACTN2, an N-terminal and a C-terminal domain, with the former domain also required for calcineurin and telethonin binding. A second N-terminal domain mediated binding to gamma-filamin. By yeast 2-hybrid analysis, they showed that ZASP (LDB3) interacted strongly with all 3 calsarcins. Preferential expression of calsarcin-3 in fast-twitch myofibers was observed using immunofluorescence.