Nucleoredoxin (NXN)

The deduced 435-amino acid protein is rich in acidic amino acids, has an internal long direct repeat, and shares significant similarity with thioredoxin (TXN). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver. In situ hybridization showed that expression of nucleoredoxin varied during mouse embryonic development. Expression was widespread in embryos at days 9.5 and 10.5, with expression in limb buds, somites, dorsal root ganglia, branchial bars, and parts of the brain, but expression was marginal at day 12.5. Epitope-tagged nucleoredoxin localized to nuclei of transfected COS-7 cells. Southern blot analysis detected nucleoredoxin orthologs in human, rat, and rabbit, but not in frog, fly, nematode, and yeast.