Peptidylprolyl Isomerase F (PPIF)

Peptidyl-prolyl cis-trans isomerase, mitochondrial is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.Northern blot analysis showed that PPIF, which the authors termed CYP3, is expressed as a major 2-kb transcript in Jurkat cells. RNA slot blot analysis revealed expression of PPIF in most cell lines and types. Western blot analysis of Jurkat cells separated by differential centrifugation suggested that PPIF is predominantly associated with membranes or organelles.