Tuftelin Interacting Protein 11 (TFIP11)

TFIP11 is a nuclear speckle-localized protein that may play a role in spliceosome disassembly in Cajal bodies ,which encodes a deduced 837-amino acid protein with a calculated molecular mass of 96.8 kD. The deduced 678-amino acid mouse protein shares 92% amino acid identity with human TFIP11. Both human and mouse proteins contain a myc-type HLH domain, at least 2 coiled-coil domains, and a C-terminal clathrin-binding motif. In situ hybridization of 1-day-old mouse mandible detected Tfip11 transcripts in secretory ameloblasts and odontoblasts. In vitro and in vivo analysis showed that Tfip11 and Tuft1 colocalized.Tfip11 colocalized with splicing factor SC35 (SFRS2), a marker for nuclear speckles, and was distinct and excluded from paraspeckles.