Centrosome And Spindle Pole Associated Protein 1 (CSPP1)

CSPP1 interacts with centrosomes and microtubules and plays a role in cell-cycle progression and spindle organization . The deduced 876-amino acid protein is highly charged and has a calculated molecular mass of 101.5 kD. CSPP1 has an N-terminal bipartite nuclear localization signal, a proline-rich region, a central domain consisting of 3 coiled-coil regions, and a C-terminal domain. Immunofluorescence studies localized CSPP1-S along microtubules, accumulating mainly around the centrosome in transfected interphase HEK293T cells. Northern blot analysis of human tissues detected a 4.4-kb transcript in testis.Immunofluorescence studies localized CSPP1-L to the microtubules and centrosomes, accumulating at the microtubule organization center in interphase, similar to CSPP1-S.