Cholinergic Receptor, Nicotinic, Beta 3 (CHRNb3)

By genomic Southern analysis of hamster/human somatic cell hybrid DNAs, Anand and Lindstrom mapped the gene encoding the beta-3 subunit of the human neuronal nicotinic acetylcholine receptor to chromosome 8. They indicated that the location of the corresponding gene in the mouse genome was not known. Koyama et al. used an exon amplification method to construct a transcriptional map of human chromosome 8. With this method, transcribed sequences from defined regions of genomic DNA could be efficiently isolated using cosmid clones mapped to chromosome 8. Sequence analysis revealed identity of a particular exon amplification fragment to the CHRNB3 gene. This transcribed fragment was isolated from a cosmid clone that had previously been mapped to 8p11.2 by fluorescence in situ hybridization.