Nerve Growth Factor Receptor Associated Protein 1 (NGFRAP1)

Using a yeast 2-hybrid screen with the cytosolic domain of p75(NTR) as bait, Mukai et al. (2000) obtained cDNAs encoding mouse and human p75(NTR)-associated cell death executor, or NADE. Human NADE is identical to the HGR74 cDNA cloned by Rapp et al. (1990) from an ovarian granulosa cDNA library. HGR74 was expressed in testis, prostate, seminal vesicle, and ovarian granulosa cells .By sequence analysis, Mukai et al. (2000) predicted that the 111-amino acid NADE protein has a leucine-rich nuclear export signal (NES) and 2 ubiquitination sequence boxes. Western blot analysis showed expression of mouse Nade only after proteasome inhibition, implying that native Nade is modified by the ubiquitin conjugating system.