Netrin G1 (NtnG1)

Netrin G1 (NTNG1) belongs to a conserved family of proteins that act as axon guidance cues during vertebrate nervous system development.The longest variant encodes a deduced 539-amino acid protein with an N-terminal signal sequence and a C-terminal glycosylphosphatidylinositol (GPI) membrane anchor. There are also 3 N-glycosylation sites. The shortest mouse Ntng1 variant appeared to correspond to KIAA0976, sharing 96% sequence identity throughout the coding region. Northern blot analysis of several adult mouse tissues detected Ntng1 only in brain. In situ hybridization of mouse embryos did not detect Ntng1 expression at embryonic day 10; expression was detected in the midbrain and hindbrain at embryonic day 12. Ntng1 expression in several brain regions and nuclei, with strongest expression in the thalamus.