Neurensin 2 (NRSN2)

Nrsn2 is broadly expressed throughout the cerebral cortex, with highest expression in the thalamus/hypothalamus. Indirect immunofluorescence in transfected Neuro2a cells showed that Nrsn2 localizes to perinuclear organelles and processes in regions distinct from those stained by Nrsn1, and in transfected COS-7 cells showed that Nrsn2 localizes to lysosomes. Immunohistochemistry in mouse brain showed similar overall distributions for Nrsn2 and Nrsn1 but different subcellular localizations, with Nrsn2 in somatic regions and Nrsn1 in neuritic processes. Nrsn2-positive cells were seen in the septum nucleus, diagonal band, hippocampus, amygdaloid nucleus, habenula nucleus, and cerebral pyriform cortex, and prominent immunostaining was seen in cell bodies of cerebellar Purkinje cells.