Nuclear Fragile X Mental Retardation Protein Interacting Protein 2 (NUFIP2)

The deduced NUFIP2 protein contains 695 amino acids and shares 95% sequence identity with the mouse protein. The protein is highly evolutionarily conserved.In the cortex, it was distributed in a diffuse way in the nucleus and in the cytoplasm. The protein was only localized in the cytoplasm in neurons of the dentate gyrus in the olfactory bulb, in the ependymal epithelium, and in the granular layer of the cerebellum. In Purkinje cells, 82-Fip was distributed in both cell compartments and was localized in nuclear dots adjacent to the nucleolus. Subcellular distribution of 82-FIP was cell cycle-dependent in cultured cells, being cytoplasmic in the G2/M phase and accumulating in nucleus during the G1 phase, which suggested that the composition of some FMRP-containing RNP complexes may be cell cycle-modulated.