Nucleolar Complex Associated 2 Homolog (NOC2L)

Histone modification by histone acetyltransferases (HAT) and histone deacetylases (HDAC) can control major aspects of transcriptional regulation. NOC2L represents a novel HDAC-independent inhibitor of histone acetyltransferase (INHAT).The 749-amino acid protein contains an N-terminal and a C-terminal INHAT domain, as well as a putative bipartite nuclear localization signal. RT-PCR analysis showed Nir mRNA present at all analyzed stages of mouse embryogenesis. Dot-blot analysis found expression in all human adult tissues tested. Northern blot analysis showed a single 3.3-kb transcript.NIR repressed basal as well as activated transcription, and HDAC inhibitors did not block NIR-mediated repression of acetylation, showing that NIR functions independent of HDAC activity.