Tubulin Gamma 2 (TUBg2)

Gamma-tubulin is an essential component of the microtubule organizing centers of eukaryotes. By screening a HeLa cell cDNA library with a sequence fragment showing homology to gamma-1-tubulin (TUBG1), Wise et al. (2000) obtained a partial TUBG2 cDNA. By EST database searching, they obtained a full-length TUBG2 cDNA clone encoding a 451-amino acid protein that shares 97.3% sequence identity with TUBG1. By RT-PCR analysis, Wise et al. (2000) detected expression of both TUBG1 and TUBG2 in all tissues examined, although at varying levels.By radiation hybrid analysis, Wise et al. (2000) mapped the TUBG2 gene to chromosome 17q21 within 20 kb of TUBG1. They mapped a TUBG2 pseudogene to chromosome 7.