Cyclin O (CCNO)

UDG2 shares no significant amino acid homology with human UNG or with uracil-DNA glycosylases from yeast, E. coli, or herpes simplex virus. Northern blot analysis detected a 1.6-kb transcript in HeLa cells. Western blot analysis detected a 36-kD protein in human fibroblasts and placenta and in monkey kidney cells, but not in hamster ovary or mouse fibroblasts.transcription of UDG2 mRNA increased 2- to 3-fold during the G1 phase of the cell cycle in both HeLa cells and human lung fibroblasts. By gel shift assays of cells prepared from various stages of the cell cycle, a repressor element in the promoter formed several specific DNA complexes during S and G2 phases that were not present during M and G1 phases. Two of the complexes resulted from binding to an inverted cell cycle box (CCB).