Downregulated In Ovarian Cancer 1 (DOC1)

FILIP1L contains an extended coiled-coil region in its N-terminal half that includes 2 leucine zipper motifs and a prefoldin domain. The N-terminal half of FILIP1L shares significant similarity with bacterial SbcC, an ATPase involved in DNA repair, and the C-terminal half of FILIP1L shares similarity with herpes virus major outer envelope glycoprotein. A second FILIP1L isoform has an N-terminal extension and contains 1,135 amino acids. Western blot analysis of fractionated human umbilical vein endothelial cells (HUVECs) detected FILIP1L at an apparent molecular mass of 110 kD predominantly in the cytoplasm, with less expression in membrane and nuclear fractions. Immunohistochemical analysis of normal human colon tissue detected FILIP1L in the vasculature and muscularis mucosa.