Far Upstream Element Binding Protein 1 (FUBP1)

FUBP1 encodes a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase.Expression of FBP in human leukemia cells stimulated the activity of a MYC promoter in a FUSE-dependent manner. Northern blot analysis revealed that expression of the 2.6-kb FBP mRNA declined upon differentiation, suggesting that FUSE-binding activity may be regulated transcriptionally. In addition, by sequence analysis of FBP cDNAs, the authors found evidence that FBP activity may also be regulated by alternative splicing, translation efficiency, and posttranslational modification.