Glioma Tumor Suppressor Candidate Region Gene 1 (GLTSCR1)

Smith et al. (2000) generated a complete transcript map of a 150-kb interval of chromosome 19q13.3 in which allelic loss is a frequent event in human diffuse gliomas. Using cDNA selection with whole brain and placenta poly(A+) RNAs, exon trapping, and genomic sequencing, they identified 2 novel transcripts, designated GLTSCR1 and GLTSCR2. The full-length coding region of GLTSCR1 was defined by skeletal muscle cDNA library screening and RT-PCR confirmation of exons predicted from genomic sequence. Northern blot analysis detected expression of a 6.5-kb GLTSCR1 transcript at moderate levels in heart, brain, placenta, skeletal muscle, and pancreas, and at lower levels in lung, liver, and kidney. Mutation analysis of the transcripts in this region in diffuse gliomas with 19q deletions revealed no tumor-specific mutations.