Glioma Tumor Suppressor Candidate Region Gene 2 (GLTSCR2)

The full-length coding region of GLTSCR2 was identified by direct cDNA selection, exon trapping, and the assembly of multiple ESTs. Northern blot analysis detected expression of a 1.5-kb GLTSCR2 transcript at high levels in heart and pancreas, moderate levels in placenta, liver, skeletal muscle, and kidney, and low levels in brain and lung.The interaction required amino acids 338 through 348 of GLTSCR2 and a C-terminal segment of PTEN that did not include the PDZ domain. Downregulation of GLTSCR2 in breast carcinoma cells by RNA interference enhanced the degradation of PTEN with concomitant decrease in PTEN phosphorylation. PTEN C-terminal tumor-associated mutants, which are highly susceptible to protein degradation, were unable to bind GLTSCR2 and showed reduced phosphorylation.