Myeloma Overexpressed Protein (MYEOV)

Sequence analysis predicted that the 313-amino acid protein contains no known functional motifs except for an RNP1 motif typical of RNA-binding proteins and a leucine-isoleucine tail similar to cytoplasmically exposed membrane proteins with a C-terminal membrane anchor.Northern blot analysis detected a major 2.8-kb and a minor 3.5-kb transcript in various tumor cell lines. In 3 of 7 multiple myeloma cell lines with a t(11;14)(q13;q32) and cyclin D1 (CCND1) overexpression, Northern blot analysis determined that MYEOV was overexpressed. In all 7 cell lines, the breakpoint was mapped to the 360-kb region between the 2 genes. MYEOV overexpression was associated with the juxtaposition of an enhancer to the MYEOV gene.